In his second round match with Casper Ruud at the final Masters 1000 event before the French Open, Kyrgios was serving to save break point early in the third set.
But the Australian mis-hit a forehand wide and conceded the break to Ruud.
Kyrgios then turned to the crowd and berated spectators for supposedly distracting him by walking around during the point.
The umpire handed Kyrgios a code violation for unsportsmanlike conduct which having already been handed a warning before led to an automatic game penalty.
Kyrgios slammed his racket into the ground, kicked a water bottle and tossed a chair across the court.
He then packed his bag, shook hands with Ruud and the umpire before walking off the court and subsequently being defaulted from the match.
Kyrgios is required to speak to the media after matches but New York Times reporter Ben Rothenberg tweeted: “ATP comms could not catch Kyrgios after the match before he left site, so, AWOL, he will not be speaking through official channels today.
“Twitter, though, likely another story.”
Kyrgios did eventually respond on Twitter with “Hahahahaha” in response to a video of Ruud celebrating his win.
But Kyrgios is unlikely to be laughing when the ATP determines his punishment.
According to the tennis rule book, Kyrgios is set to receive significant action against him.
As a result of his default, Kyrgios loses all the prize money, hotel accommodations and ATP points earned from the event.
Had Kyrgios been in the doubles, the tournament supervisor has the discretion to remove him from the event.
And if the Executive Vice President finds that Kyrgios’ behaviour was “particularly injurious to the success of the tournament or detrimental to the integrity of the sport, he may consider additional penalties (fines and/or suspensions).
Kyrgios has a history poor behaviour on the court including making an insulting remark to Stan Wawrinka which led to a £16,200 fine and breaching the ATP code of conduct after arguing and swearing at a fan.